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Spring Cleaning of Microbiology Lab

posted by ichappsseo 2 days ago
tags: Microbiology Spring Cleaning

Put off all the instrument and cover them with polyethylene sheet. Clean the area thoroughly including walls, ceiling, Doors and windows, light fixers with dry mop or vacuum cleaner. Start the cleaning of rooms from critical to non-critical area.

https://ichapps.com/qms/view/spring-cleaning-of-microbiology-lab

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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above said beaker, through it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel.

https://ichapps.com/qms/view/preparation-of-mercuric-acetate-solution-10-percent

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Procedure for sampling and analysis of Raw material and Finished product for microbial analysis

Sampling shall be done for microbial analysis for regular batches as per SOP XXXXX. Sampling shall be done by microbiologist only for product validation by following the below procedure. Requirement Sterile Glass bottles / sterile polyethylene bags Sterile Spatula / Sampling Rod.

https://ichapps.com/qms/view/procedure-for-sampling-and-analysis-of-raw-material-and-finished-product-for-microbial-analysis

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Procedure for Receipt and Issuance of Endotoxin testing Materials

Take the sample (product diluted to 2-fold of MVD) 50?l each in endotoxin free tubes by micropipette fitted with disposable tip. Keep all the sets in duplicate for product positive and product negative. Add 50?l of reconstituted Control Standard Endotoxin diluted to 2? in positive tubes and 50?l of LAL reagent water in negative tubes.

https://ichapps.com/qms/view/procedure-for-receipt-and-issuance-of-endotoxin-testing-materials-and-testing-of-endotoxins-in-finished-product

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PREPARATION OF NITRIC ACID DILUTE (10 percent)

Measure 10.6 ml of Nitric Acid LR and transfer into a 50.0 ml capacity beaker. Pour about 75.0 ml of distilled water into a 250 ml capacity beaker. Transfer slowly, through sidewalls of the beaker, 10.6 ml of Nitric Acid LR into above said beaker, while gentle mixing, by using a glass rod.

https://ichapps.com/qms/view/preparation-of-nitric-acid-dilute-10-percent

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Procedure for Disinfectant Efficacy Test

Pure cultures of Staphylococcus aureus xxxxxnas aeruginosa xxxxx Sterilized Conical flasks (250 ml capacity) Sterilized graduated pipettes (1 ml). Sterilized Petriplates. Sterilized test tubes (18 x 150 mm). Nutrient agar / Soya bean casein digest agar. Nutrient broth / Soya bean casein digest medium. Inactivating agent (Lecithin 0.5% with tween 20 4.0% / Sodium thiosulfate / Sodium thioglycollate)

https://ichapps.com/qms/view/procedure-for-disinfectant-efficacy-test

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Procedure for Finger Dab Test

Prepare soyabean casein digest agar plate as per the current version of general test procedure No. GTP-210. Take the solidified agar plates in sterile Stainless Steel perticans and carry it to respective area for finger dab test. Wear sterile hand gloves, open the box and remove the plates. Carefully open the plate and touch the finger and thumb of operator / microbiologist on to the agar surface, who is directly in contact with product/ analysis.

https://ichapps.com/qms/view/procedure-for-finger-dab-test

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PREPARATION OF NITRIC ACID DILUTE

Measure 53.0 ml of Nitric Acid LR and transfer into a 100 ml capacity beaker. Pour about 200 ml of distilled water into a 500 ml capacity beaker. Transfer slowly, through sidewalls of the beaker, 53.0 ml of Nitric Acid LR (about 10ml on each attempt) into above said beaker, while gentle mixing, by using a glass rod.

https://ichapps.com/qms/view/preparation-of-nitric-acid-dilute

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Procedure for Operation Cleaning and Calibration of Bacteriological Incubators

Connect power plug to the socket. Put ON the Mains on the control panel, Orange lamp inside it glows and temperature controller as well as the PC / Interface displays and the inside Incubator motor ON. Put ON the heating switch on the control panel, Dry heater will ON and green indicator lamp glows.

https://ichapps.com/qms/view/procedure-for-operation-cleaning-and-calibration-of-bacteriological-incubators

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pPreparation of silver nitrate solution

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Silver Nitrate LR and transfer slowly, into above said beaker, through it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask.

https://ichapps.com/qms/view/preparation-of-silver-nitrate-solution-5-percent

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